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The cell membranes pose a strong barrier for entry of extraneous molecules into cells and devising strategies to overcome this barrier is one of the most challenges in drug delivery.Several cationic peptides are known to be effective as cell penetrating agents.In this lecture our recent efforts to employ cationic collagen peptide analogues P1-P4 (X-Y-Gly)n,where X/Y are 4(R/S)-arnino and 4(R/S)-guanidino prolines for transfection of plasmid DNA encoding green fluorescent protein will be demonstrated.Of interest are the stereochemical dependence of 4(R/S) substitutions on cell penetration and a better transfection efficiency of cationic peptides compared to commercial reagents.Similar strategies to enable peptide nucleic acids enter cells by making them cationic analogues(PNA 1-3) will be presented.