【摘 要】
：
At first,we optimized separation protocol of membrane proteins of Aspergillus fumigates by 2D Fluorescence difference gelelectrophoresis (2D DIGE).We found that membrane proteins were dissolved well i
【机 构】
：
State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences
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At first,we optimized separation protocol of membrane proteins of Aspergillus fumigates by 2D Fluorescence difference gelelectrophoresis (2D DIGE).We found that membrane proteins were dissolved well in followed lysis buffer:7Murea,4％CHAPS,2Mthiourea,1％ASB14,30mMTris,2％ TritonX-100,1％DTT,1％IPG buffer.According to this effective 2D DIGE procedure,weresearched the differential expression of membrane proteins among the wild,pmt1 and pmt2 whose mannose transferase wasknocked out.
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