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  5. Mechanism of Eaf2 Gene Knockout Regulating microRNA in the Pathogenesis of Cataract

Mechanism of Eaf2 Gene Knockout Regulating microRNA in the Pathogenesis of Cataract

来源 :第八届中国眼科学和视觉科学研究大会 | 被引量 : 0次 | 上传用户：jsjyao
【摘 要】
：
Purpose To explore the expression of microRNA in Eaf2 knockout mouse,and the effect of Eaf2 on both human lens epithelial cell apoptosis and the expression of microRNA in lens.Methods pEGFP-C1-Eaf2 wa
【作 者】
：
Qin Yu Zhang Jinsong Zhao Jiangyue 
【机 构】
：
Eye Hospital of China Medical University Departme
【出 处】
：
第八届中国眼科学和视觉科学研究大会
【发表日期】
：
2016年5期
【关键词】
：
Eaf2 knockout microRNA gene regulation cataract 
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　　Purpose To explore the expression of microRNA in Eaf2 knockout mouse,and the effect of Eaf2 on both human lens epithelial cell apoptosis and the expression of microRNA in lens.Methods pEGFP-C1-Eaf2 was transfected into SRA01/04 cells using Lipofectamine 2000 to over express the expression of Eaf2 gene,and then flow cytometry was used to detect cell apoptosis rate.
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