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目的:探讨肿瘤坏死因子相关诱导凋亡配体(TRAIL)与紫杉醇联合应用对喉鳞状细胞癌细胞Hep-2的抑制作用。方法:CCK-8测定TRAIL和紫杉醇对Hep-2细胞的生长抑制率,流式细胞术检测细胞表面TRAIL受体的表达及细胞凋亡。结果:Hep-2细胞对TRAIL诱导的凋亡不敏感,紫杉醇通过上调细胞表面死亡受体的表达而增强Hep-2细胞对TRAIL的敏感性。结论:紫杉醇可使Hep-2细胞克服对TRAIL的耐受性,两者具有协同杀伤效应,有望应用于喉鳞状细胞癌的临床治疗。
Objective: To investigate the inhibitory effect of combination of TRAIL and paclitaxel on Hep-2 cells in laryngeal squamous cell carcinoma. Methods: The inhibitory rate of TRAIL and paclitaxel on Hep-2 cells was determined by CCK-8. The expression of TRAIL receptor and cell apoptosis were detected by flow cytometry. Results: Hep-2 cells were not sensitive to TRAIL-induced apoptosis. Paclitaxel enhanced the sensitivity of Hep-2 cells to TRAIL by up-regulating the expression of cell surface death receptors. Conclusion: Paclitaxel can overcome the resistance of Hep-2 cells to TRAIL. Both of them have a synergistic killing effect and are expected to be used in the clinical treatment of laryngeal squamous cell carcinoma.