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目的 了解恶性疟原虫云南株 PFD- 3/ YN pfs2 5基因序列变异情况。方法 PCR扩增恶性疟原虫云南株 PFD- 3/YN株 pfs2 5全基因 ,经 Sanger双脱氧链终止法测序和 Pst I酶切鉴定 ,并与国外恶性疟原虫 NF4株 pfs2 5进行比较。结果 恶性疟原虫云南株 PFD- 3/ YN株 pfs2 5基因与 NF4株的 pfs2 5基因其同源性为 99.2 %。结论 PFD- 3/ YN株与 NF4株 pfs2 5基因表现很高的同源性
Objective To understand the sequence variation of PFD-3 / YN pfs2 5 gene in P. falciparum Yunnan strain. Methods The pfs2 5 gene of P. falciparum strain PFD-3 / YN was amplified by polymerase chain reaction (PCR) and identified by Sanger dideoxy chain termination method and Pst I restriction enzyme digestion. The results were compared with the pfs2 5 of P. falciparum NF4 strain. Results The pfs2 5 gene of PFD-3 / YN strain of P. falciparum isolates had a homology of 99.2% with the pfs2 5 gene of NF4 strain. Conclusion The pfs2 5 gene of PFD-3 / YN strain and NF4 strain showed high homology