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【目的】比较松油烯-4-醇光学异构体对家蝇Musca domestica的熏蒸活性差异,为其光学异构体的应用提供理论依据。【方法】以家蝇4日龄成虫为供试昆虫,采用三角瓶熏蒸法比较测定了松油烯-4-醇光学异构体和外消旋体对其的熏蒸与击倒活性,并测定了松油烯-4-醇光学异构体及外消旋体对家蝇头部Na+,K+-ATPase活性的影响。【结果】松油烯-4-醇外消旋体对家蝇的熏蒸活性和击倒活性最强,右旋异构体次之,左旋异构体最差,外消旋体、右旋异构体和左旋异构体对家蝇的致死中浓度(LC50)分别为2.5,2.9和3.7μL/L;在LC90剂量下的击倒中时(KT50)分别为12.6,16.7和18.9 min;松油烯-4-醇光学异构体及外消旋体均可显著抑制Na+,K+-ATPase的活性,活体条件下,松油烯-4-醇光学异构体及外消旋体对Na+,K+-ATPase活性的抑制作用随着中毒症状的加剧而增强,具有时间效应,其中左旋异构体的抑制作用最强;离体条件下,松油烯-4-醇光学异构体及外消旋体对Na+,K+-ATPase活性的抑制作用具有浓度依赖效应,其中外消旋体对Na+,K+-ATPase活性的抑制能力最强,明显高于同浓度下的右旋异构体和左旋异构体。【结论】松油烯-4-醇的光学异构体对家蝇的杀虫活性存在差异,外消旋体的活性明显高于异构体单体。开发松油烯-4-醇类杀虫剂,应以光学异构体的混合物作为有效成分。
【Objective】 The aim of this study was to compare the fumigating activities of the optical isomers of Terpen-4-ol with Musca domestica and to provide a theoretical basis for the application of optical isomers. 【Method】 Four-day-old adults of housefly were used as test insects, and the fumigant and knockdown activities of optical isomers and racemates of terfenn-4-ol were determined by the flask fumigation method. Effect of optical isomers and racemates of terpentene - 4 - ol on Na +, K + -ATPase activity in housefly heads. 【Result】 The results showed that the activity of knockout and activity of terpinen-4-ol racemate were the strongest among them, the dextral isomer followed by the dextral isomer, the levorotatory isomer the worst, the racemate and dextrorotatory The lethal concentrations (LC50) of the structures and levorotatory isomers to house flies were 2.5, 2.9 and 3.7 μL / L, respectively; the KT50 at the LC90 dose was 12.6, 16.7 and 18.9 min respectively; O-enol-4-ol optical isomers and racemates can significantly inhibit the activity of Na +, K + -ATPase. Under living conditions, the optical isomers and racemates of terpinen-4- The inhibitory effect of K + -ATPase activity increased with the aggravation of the symptoms of poisoning, with the time effect, in which the inhibitory effect of levorotatory is the strongest; in vitro, terpinen-4-ol optical isomers and exogenous The inhibition of Na +, K + -ATPase activity by spinosad has a concentration-dependent effect, in which racemate has the strongest inhibitory activity on Na +, K + -ATPase activity, which is significantly higher than that of dextrorotatory and levorotatory at the same concentration Structure. 【Conclusion】 The insecticidal activity of optical isomers of terpinen-4-ol toward house flies is different. The racemate activity is significantly higher than that of isomer monomers. The development of terpentene-4-alcohol insecticide should be a mixture of optical isomers as an active ingredient.