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目的对十堰市5类场所的标本进行禽流感病毒特异性核酸检测,为禽流感的感染来源调查提供方向与线索。方法在2009-2012年间,选择十堰市5类场所,按季度采集不同种类的标本675份,采用Real-time PCR方法对标本进行禽流感病毒特异性核酸检测,结果使用SPSS17.0进行分析。结果FluA在野生候鸟栖息地、活禽农贸市场、大型家禽屠宰厂、大型家禽养殖场的检出率分别为1.41%、66.36%、51.72%、5.26%,集中散养户未检测到阳性标本,5类场所检出率存在统计学差异(χ2104.581,P0.05);FluA在禽类内脏、冲洗内脏水及污水、粪便、鸡舍(笼)、死禽及蛋壳中的检出率分别为47.79%、32.86%、13.28%、19.84%、1.28%,禽类饮用水中未检测到,不同种类标本中FluA的检出率有统计学差异(χ257.304,P0.05)。结论不同种类的标本检出率从高到低依次为:禽内脏、冲洗内脏水及污水、粪便、鸡笼、死禽及蛋壳、饮用水等。
Objective To detect the specific nucleic acid of avian influenza virus in five kinds of places in Shiyan City and provide the direction and clues for the investigation of the source of bird flu infection. Methods Between 2009 and 2012, 5 places in Shiyan City were selected, and 675 specimens of different species were collected on a quarterly basis. Real-time PCR was used to detect the avian influenza virus-specific nucleic acids in the specimens. The results were analyzed by SPSS17.0. Results The detection rates of FluA in wild migratory birds habitat, live poultry farmer’s market, large poultry slaughterhouse and large-scale poultry farm were 1.41%, 66.36%, 51.72% and 5.26% respectively. No positive samples were detected in concentrated migratory households, There was a significant difference in the detection rates of 5 kinds of places (χ2104.581, P <0.05). The detection rates of FluA in viscera, viscera and sewage, feces, house (cage) (47.79%, 32.86%, 13.28%, 19.84%, 1.28%, respectively). FluA was not detected in poultry drinking water. The detection rates of FluA in different types of samples were statistically different (χ257.304, P <0.05). Conclusions The detection rates of different types of specimens are as follows: viscera of poultry, viscera and sewage flushing, excrement, cages, dead poultry and eggshells, drinking water and so on.