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为了探讨建立毒死蜱残留检测方法,研究人员以毒死蜱原药与3-巯基丙酸为起始原料,合成半抗原并对其进行结构鉴定。利用该半抗原与牛血清蛋白(BSA)和卵清蛋白(OVA)偶联制备毒死蜱人工免疫抗原和包被抗原,再取已免疫的经过筛选的Balb/c小鼠脾细胞与小鼠骨髓瘤细胞SP2/0融合,用间接ELISA和间接竞争ELISA方法
In order to explore the establishment of chlorpyrifos residue detection method, the researchers chlorpyrifos original drug and 3-mercaptopropionic acid as the starting material, synthesis of haptens and its structural identification. The hapten was used to conjugate bovine serum albumin (BSA) and ovalbumin (OVA) to prepare artificial immune antigen and coated antigen, and then the immunized selected Balb / c mouse spleen cells and mouse myeloma Cells were SP2 / 0 fused with indirect ELISA and indirect competitive ELISA