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目的 观察眼镜蛇毒及其组分 Ｃ 抗小鼠肝癌的病理学改变。 方法 采用不同剂量眼镜蛇毒及其抗癌活性组分 Ｃ 与 Ｂ Ａ Ｌ Ａ／ｃ 小鼠腹水型肝癌细胞体外孵育， 空白对照组用生理盐水与肝癌细胞孵育， 然后取孵育液接种于小鼠前肢腋下， 接种后第 １０ｄ 处死， 解剖取出瘤结， 进行病理组织学研究。 结果 空白对照组瘤结较大， 显微镜下见瘤细胞生长活跃、核大、核仁明显、核分裂多见， 而坏死灶少见， 且瘤细胞向周围浸润扩散； 治疗组瘤体较小， 瘤细胞固缩、核仁不明显、核分裂少见， 而坏死灶多见， 瘤细胞周围有纤维组织增生围绕， 限制了瘤细胞向周围蔓延浸润。 结论 眼镜蛇毒及其组分 Ｃ 对小鼠实验性肝癌的体外抗癌作用是明显的， 不同剂量及不同孵育时间的抗癌作用亦显著不同， 其中组分 Ｃ 对抗癌作用最强。
Objective To observe the pathological changes of cobra venom and its component C against liver cancer in mice. Methods Different doses of cobra venom and its anticancer active component C and B A L A/c mouse ascites hepatoma cells were incubated in vitro. The blank control group was incubated with normal saline and hepatoma cells, and then the incubation solution was used to inoculate the mouse forelimbs. The armpits were sacrificed on the 10th day after inoculation. The tumors were dissected and histopathologically studied. RESULTS: In the control group, the neoplasia was large. Under the microscope, the tumor cells grew actively, the nucleus was large, the nucleoli was obvious, and the nuclear fission was more common. The necrotic lesions were rare, and the neoplastic cells spread to the surrounding infiltration; The tumor cells in the treatment group were small, and the tumor cells were small. Pyriacosis, nucleoli inconspicuous, rare mitoses, and necrosis foci more common around the tumor cells around the proliferation of fibrous tissue, limiting the spread of tumor cells to the surrounding infiltration. Conclusion The cobra venom and its components C have significant anti-cancer effects on experimental liver cancer in mice. The anticancer effects of different doses and different incubation times are also significantly different. Component C has the strongest anticancer effect.