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输血是乙型肝炎病毒(HBV)传播的重要途径之一。据WHO综合各国资料表明,输血后乙型肝炎发生率为3.7‰,这与筛选献血员方法的灵敏性有很大关系。本文分别应用被动血凝(RPHA)、放射免疫分析(RIA)、酶联免疫吸附试验(ELISA)、HBVDNA探针及聚合酶链反应(PCR)技术对150例献血员血清中HBV的感染状况进行了观察,现将结果报告如下。材料和方法一、材料 (一)被检对象:本院献血员150例,男104例、女46例,年龄28～48岁,献血年限5～10年。 (二)献血员空腹抽血3ml,立即分离血清置低温冰箱备检。 (三)试剂 1.HBV-PCR引物由上海肿瘤研究所提供;FD-DNA聚合酶由复旦大学遗传所提供;dNTP由
Blood transfusion is one of the important ways of transmitting hepatitis B virus (HBV). According to WHO data from various countries, the incidence of hepatitis B after blood transfusion is 3.7 ‰, which is closely related to the sensitivity of screening blood donors. In this paper, the status of HBV infection in 150 blood donors was studied by using passive hemagglutination (RPHA), radioimmunoassay (RIA), enzyme-linked immunosorbent assay (ELISA), HBVDNA probe and polymerase chain reaction Observed, the results are reported below. MATERIALS AND METHODS I. MATERIALS (I) Subjects: 150 blood donors, 104 males and 46 females, aged from 28 to 48 years and blood donation years of 5 to 10 years. (B) blood donors fasting blood 3ml, immediately separated serum cryogenic refrigerator for inspection. (C) Reagents 1. HBV-PCR primers provided by the Shanghai Cancer Institute; FD-DNA polymerase provided by the Fudan University Institute of Genetics; dNTP by