论文部分内容阅读
目的:探讨罗格列酮(ROZ)对体外培养细胞系LNCAP增殖及凋亡的作用。方法:20、50、100μmol/LROZ作用LNCAP细胞24h、48h、72h后,MTT法检测R0Z对LNCAP细胞生长的影响。采用末端脱氧核苷酸转移酶原位标记(TUNEL法)和流式细胞术检测细胞凋亡情况。Western Blot检测LNCAP细胞中Casepase-3表达水平的变化。结果:ROZ(20μmol/L至100μmol/L)对前列腺癌细胞有显著的增殖抑制作用,以100μmol/L浓度最为明显。ROZ(100μmol/L)作用72h后最高增殖抑制率达78.58%。流式细胞仪检测结果显示,LNCAP细胞凋亡数目随药物浓度的增加呈递增趋势;100μmol/LROZ作用72h后凋亡细胞数达48.11%。与此一致,TUNEL染色阳性细胞数目在ROZ处理组显著增多,WesternBlot检测显示,ROZ显著增加LNCAP细胞中Caspase-3蛋白表达水平。结论:ROZ在体外对前列腺癌细胞系LNCAP有明显的增殖抑制及促进凋亡作用,有望成为前列腺癌患者新的治疗方法。
Objective: To investigate the effect of rosiglitazone (ROZ) on proliferation and apoptosis of cultured LNCAP cell line. Methods: LNCAP cells were treated with 20, 50 and 100μmol / LROZ for 24h, 48h and 72h, respectively. The effect of R0Z on the growth of LNCAP cells was detected by MTT assay. Apoptosis was detected by TUNEL and flow cytometry. Western Blot detection of LNCAP cells Casepase-3 expression levels. Results: ROZ (20μmol / L to 100μmol / L) significantly inhibited the proliferation of prostate cancer cells, with the concentration of 100μmol / L being the most obvious. The highest proliferation inhibition rate of ROZ (100μmol / L) for 72h was 78.58%. The results of flow cytometry showed that the number of apoptotic LNCAP cells increased with the increase of drug concentration, and the number of apoptotic cells reached 48.11% at 72h after treated with 100μmol / LROZ. Consistent with this, the number of TUNEL positive cells in ROZ treatment group increased significantly, Western blot analysis showed that ROZ significantly increased the expression of Caspase-3 protein in LNCAP cells. CONCLUSION: ROZ can inhibit proliferation and promote apoptosis of prostate cancer cell line LNCAP in vitro, which is expected to become a new treatment for patients with prostate cancer.