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本文就51例非胰岛素依赖性糖尿病患者的红细胞钠泵活性、细胞膜脂区流动性及血清脂质浓度之间的相互联系及与糖尿病的关系,作一初步探讨。 方法 ①红细胞钠泵活性用Lasise改良法;②红细胞膜脂区流动性(以荧光偏振度P和微粘度(?)表示)以DPH(1,6-二苯基-1,3,5-己烯)为荧光探剂标记红细胞膜脂区;③血清脂质浓度用酶法,分别测定胆固醇(TC)、高密度脂蛋白(HDL-C)及其亚组分(HDL_2-C及HDL_3-C)。 结果 ①25例糖尿病患者的红细胞钠泵活性为每小时1.502±0.07mmol/L RBC(m±SE,下同),与10例正常对照组(1.141±0.07)相比,p<0.001;②41例糖尿病患者的荧光偏振度(p)为0.284±0.003,平均微粘度(?)为3.293±0.115,与20例正常对照组(p 0.245±0.002,(?)2.282±0.039)比较,p<0.001;③血清脂质浓度:51例患者的TC为4.34±0.16mmol/L,明显高于正常对照组(3.94±0.07,n=82);糖尿病组HDL-C为1.13±0.04mmol/L、HDL_2-C为0.31±0.02mmol/L,明显低于正常对照组(HDL-C为1.28±0.02、HDL_2-C 0.49±0.02),p值分别<0.005及<0.001。HDL_3-C浓度两组无显著差别。
In this paper, 51 cases of non-insulin-dependent diabetic patients with erythrocyte sodium pump activity, cell membrane lipid mobility and serum lipid levels and their relationship with the relationship between diabetes for a preliminary study. Methods ① The sodium pump activity of erythrocytes was modified by Lasise method; ② The fluidity of erythrocyte membrane lipid region (expressed by fluorescence polarization degree P and microviscosity (?)) Was measured by DPH (1,6-diphenyl- (TC), high density lipoprotein (HDL-C) and its sub-components (HDL-2-C and HDL-3-C ). Results ① The sodium pump activity of erythrocytes in 25 diabetic patients was 1.502 ± 0.07mmol / L RBC (m ± SE) per hour, p <0.001 compared with 10 normal controls (1.141 ± 0.07) The fluorescence polarization (p) was 0.284 ± 0.003 and the average microviscosity was 3.293 ± 0.115, p <0.001 compared with 20 normal controls (p 0.245 ± 0.002, 2.282 ± 0.039) Serum lipid concentrations: 51 patients TC was 4.34 ± 0.16mmol / L, significantly higher than the normal control group (3.94 ± 0.07, n = 82); HDL-C was 1.13 ± 0.04mmol / L, HDL_2-C Was 0.31 ± 0.02mmol / L, significantly lower than the control group (HDL-C was 1.28 ± 0.02, HDL_2-C 0.49 ± 0.02), p values were <0.005 and <0.001 respectively. There was no significant difference in HDL_3-C concentrations between the two groups.