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通过对番茄成熟突变体和野生型果实香气物质、乙烯释放量、氢过氧化物裂解酶(HPL)、脂氧合酶(LOX)活性及基因表达的测定,明确LOX与乙烯在番茄果实香气物质合成中的作用。以番茄成熟突变体rin、nor、cnr、nr(均为乙烯合成缺陷突变体)、hp-1(高色素突变体,乙烯生成正常)和野生型Ailsa Craig(AC)为试材,分别在果实破色期(0 d)、破色3 d(3 d)和破色7 d(7 d)取果实,测定香气物质、乙烯释放量、LeHPL基因表达、LOX活性和TomloxC基因表达。结果表明,随着果实的成熟,4种乙烯合成突变体果实中的香气物质种类和含量与野生型AC相比均减少,但突变体hp-1果实中香气物质与AC无显著差异;rin、nor、cnr突变体中不产生乙烯,nr中产生少量乙烯,hp-1和AC果实中能正常生成乙烯;除AC果实外,突变体rin、nor、cnr果实中LOX酶活性和TomloxC基因表达水平均较低,而在hp-1和nr破色7 d果实中酶活性和基因表达量均最高;Le HPL表达量在AC和hp-1果实中随果实成熟显著增加,在nor破色7 d显著高于破色期(0 d)和破色3 d的,而在cnr、rin和nr破色7 d均显著下降。在乙烯合成缺陷突变体果实中香气物质种类和含量较野生型减少,且不能合成乙烯或合成少量乙烯,同时LOX酶活性降低,TomloxC及LeHPL表达下降,表明番茄果实香气物质合成的LOX途径是依赖乙烯的过程。
Through the determination of the aroma components, ethylene emission, HPL, LOX and gene expression of tomato mature mutants and wild-type fruits, the effects of LOX and ethylene on tomato fruit aroma substances The role of synthesis. In this study, rin, nor, cnr, nr (all mutants defective in ethylene synthesis), hp-1 (hyperchrombin mutant, normal ethylene production) and wild type Ailsa Craig (AC) Aromatic substances, ethylene emission, LeHPL gene expression, LOX activity, and TomloxC gene expression were determined by colorimetry (0 d), broken color for 3 d (3 d) and broken color for 7 d (7 d). The results showed that with the maturation of fruits, the aroma substances in the fruits of the four ethylene-producing mutants decreased compared with the wild-type AC, but there was no significant difference between the aroma substances and the AC in the mutant hp-1 fruit; rin, Nor and cnr mutants did not produce ethylene, nr produced a small amount of ethylene, hp-1 and AC fruit can produce ethylene normally; in addition to AC fruit, the mutant rin, nor, cnr fruit LOX enzyme activity and TomloxC gene expression levels Were lower, while the activity and gene expression of the fruit were the highest in the hp-1 and nr crushed fruit for 7 d. The expression level of Le HPL in fruits of AC and hp-1 increased significantly with fruit ripening, (0 d) and broken color for 3 d, while the decolorization of cnr, rin and nr decreased significantly on the 7th day. In the ethylene-deficient mutant, the type and content of the aroma substances decreased compared with the wild type, and ethylene could not be synthesized or a small amount of ethylene could be synthesized. Meanwhile, the LOX enzyme activity decreased and the expression of TomloxC and LeHPL decreased, indicating that LOX pathway of tomato fruit aroma substance synthesis was dependent Ethylene process.