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作者鉴定了一种新的热休克基因,称为htpY,位于dnaK duaJ操纵子上游700bp。htpY基因产物显示正性介导热休克基因的表达,因为无htpY突变导致热休克基因表达减少,而过量htpY蛋白则引起热休克基因表达水平升高。某种dnaK突变型细菌可能引起一个附加的突变或者dnaK dnaJ图谱上游的多点突变,这种dnaK突变型细菌的温度敏感性原型归于推定的突变基因htpY,它是在高温条件下为细菌生长所需的,意味这个基因编码热休克蛋白。要克隆推定的ht-pY基因,首先从大肠杆菌λ101噬菌体DNA库克隆一条7.5kbBamHI酶切片段到低拷
The authors identified a new heat shock gene, called htpY, located 700bp upstream of the dnaK duaJ operon. The htpY gene product shows a positive induction of heat shock gene expression because no htpY mutation leads to a decrease in heat shock gene expression whereas excessive htpY protein causes an increase in heat shock gene expression. A certain dnaK mutant bacterium may cause an additional mutation or a multipoint mutation upstream of the dnaK dnaJ map. The temperature-sensitive prototype of this dnaK mutant bacterium is due to the putative mutant gene htpY, which is a bacterial growth site under high temperature Needed, meaning that this gene encodes a heat shock protein. To clone the putative ht-pY gene, a 7.5 kb BamHI fragment was first cloned from the E. coli lambda 101 phage DNA library to low copy