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Forss-Petter等最近报告了揭示神经元特异性基因表达中调节机制的初步工作。因为所有的成熟神经元都表达神经元特异性烯醇酶(NSE),所以人们都期望NSE基因的转录调节因子对所有的神经元特异性基因都是相同的。现已发现这些调节因子位于NSE基因密码区域上方的1.8-Kb序列之中。为了确定这个序列是否能够在体内授予神经元特异性转录以活性,Forss-Petter等培养了移植基因小鼠(tranlsgenic mice),它们携带有由NSE基因的1.8-Kb之5′侧翼序列与细菌的β-半乳糖苷酶编码序列相熔合的一个杂交基因。
Forss-Petter et al. Recently reported preliminary work to reveal regulatory mechanisms in neuron-specific gene expression. Because all mature neurons express neuron-specific enolase (NSE), one would expect that the transcriptional regulator of the NSE gene will be the same for all neuron-specific genes. It has been found that these regulators are located in the 1.8-Kb sequence above the NSE gene’s coding region. In order to determine if this sequence is capable of conferring neuronal specific transcriptional activity in vivo, Forss-Petter et al. Cultured tranlsgenic mice carrying the 5 ’flanking sequence of 1.8-Kb of NSE gene with bacterial A hybrid gene fused to the beta-galactosidase coding sequence.