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目的:建立西他沙星及其异构体高效毛细管电泳拆分的新方法。方法:以β-环糊精及几种环糊精衍生物作为手性添加剂,采用毛细管电泳分离新型抗菌素西他沙星与其3个异构体。对运行缓冲液的浓度、pH,环糊精的种类、二元环糊精体系中环糊精的比例,分离电压,柱温,有机改性剂等因素对分离的影响进行考察,并对拆分机制进行初步探讨。结果:运行缓冲液为含2%硫酸化-β-环糊精和1%β-环糊精的25 mmol.L-1的磷酸二氢钾-磷酸缓冲溶液(pH 2.5),操作电压为-25kV,柱温为25℃,检测波长为295 nm,西他沙星与其3个异构体达到良好分离。结论:所建立的毛细管电泳方法简便,适用于西他沙星异构体的检查。
Objective: To establish a new method of high performance capillary electrophoresis separation of xyloflorate and its isomers. Methods: β-Cyclodextrin and several cyclodextrin derivatives were used as chiral additives, and the novel antibiotic sitaxatoxin and its three isomers were separated by capillary electrophoresis. The influences of the factors such as the concentration of running buffer, pH, the type of cyclodextrin, the ratio of cyclodextrin in binary cyclodextrin system, the separation voltage, the column temperature and the organic modifier were investigated. Mechanism to conduct a preliminary discussion. Results: The running buffer was 25 mmol.L-1 potassium dihydrogenphosphate-phosphate buffer (pH 2.5) containing 2% sulfated β-cyclodextrin and 1% β-cyclodextrin. The operating voltage was - 25 kV, the column temperature was 25 ℃ and the detection wavelength was 295 nm. Sitafloxacin and its three isomers were well separated. Conclusion: The established capillary electrophoresis method is simple and suitable for the determination of isofloxacin isomers.