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用ＬＳＡＢ免疫组化法，对２０例胃癌、２２例异型增生、３０例肠化生及１３例正常组织进行了ｒｓａ－ｐ２１和突变型ｐ５３蛋白表达的检测。结果发现，胃癌、异型增生及肠化生，ｒａｓ－ｐ２１和ｐ５３阳性率较高，正常组织全部阴性；在所有病变组织中，ＨＰ阳性病人的ｐ２１和ｐ５３阳性率分别为４１．６％（３０／７２）和２７．７％（２０／７２），明显高于ＨＰ阴性病人，有统计学意义（Ｐ＜０．０１；Ｐ＜０．０５）。结果表明，ＨＰ感染与ｒａｓ－ｐ２１和突变型ｐ５３过度表达存在着明显的相关性，提示ＨＰ感染参予了ｒａｓ原癌基因激活和ｐ５３抑癌基因的失活，基因突变可能是ＨＰ致胃癌作用的机制之一。
Using LSAB immunohistochemical method, the expression of rsa-p21 and mutant p53 protein was detected in 20 cases of gastric cancer, 22 cases of dysplasia, 30 cases of intestinal metaplasia, and 13 cases of normal tissues. The results showed that the positive rates of ras-p21 and p53 were higher in gastric cancer, dysplasia and intestinal metaplasia, and all were negative in normal tissues. In all lesions, the positive rate of p21 and p53 in HP positive patients was 41.6% (30). /72) and 27.7% (20/72) were significantly higher than HP negative patients, with statistical significance (P<0.01; P<0.05). The results showed that there was a significant correlation between HP infection and the over-expression of ras-p21 and mutant p53, suggesting that HP infection was involved in the activation of ras proto-oncogene and the inactivation of p53 tumor suppressor gene. The gene mutation may be the role of HP in gastric carcinogenesis. One of the mechanisms.