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[目的]检测大鼠肝脏组织中MEF2A/2C mRNA及HDAC4/10 mRNA表达水平,并分析MEF2A/2C mRNA与HDAC4/10 mRNA表达的相关性。[方法]采用0.01%二乙基亚硝胺饲喂Wistar大鼠,建立大鼠肝癌模型,提取大鼠肝组织中的总RNA并逆转录为cDNA,应用Real-timePCR检测MEF2A/2C mRNA与HDAC4/10 mRNA表达量。[结果]18周后,大鼠致癌率为100%,总死亡率为35%,MEF2A/2C mRNA在第4、8周表达量显著增加(P<0.01),HDAC4/10mR-NA在第4周大量表达(P<0.01),随后都逐渐趋于正常水平。MEF2A/2C mRNA与HDAC4/10 mRNA表达水平在时间上不存在相关性。[结论]大鼠肝癌发生过程中,MEF2与肝癌组蛋白乙酰化有关,但MEF2A/2C mRNA与HDAC4/10 mRNA表达量在时间上无明显的关联性。
[Objective] To detect the expression of MEF2A / 2C mRNA and HDAC4 / 10 mRNA in rat liver tissue and analyze the correlation between MEF2A / 2C mRNA and HDAC4 / 10 mRNA expression. [Method] Wistar rats were fed with 0.01% diethylnitrosamine to establish a rat model of hepatocellular carcinoma. Total RNA was extracted from rat liver tissue and reverse transcribed into cDNA. Real-time PCR was used to detect the expression of MEF2A / 2C mRNA and HDAC4 / 10 mRNA expression level. [Results] After 18 weeks, the carcinogenicity of rat was 100% and the total mortality was 35%. The expression of MEF2A / 2C mRNA in the 4th and 8th week was significantly increased (P <0.01), HDAC4 / 10mR-NA in the 4th Week (P <0.01), then gradually normalized. MEF2A / 2C mRNA and HDAC4 / 10 mRNA expression levels in the time there is no correlation. [Conclusion] MEF2 is associated with histone acetylation in HCC during rat hepatocarcinogenesis, but the expression of MEF2A / 2C mRNA and HDAC4 / 10 mRNA have no obvious correlation in time.