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目的:建立SD大鼠运动预处理模型和一次性力竭运动模型,探讨运动预处理对一次性力竭运动诱导的大鼠大脑皮质神经胶质酸性蛋白表达的影响。方法:将30只SD雄性大鼠随机分为3组:对照组(C组),力竭运动组(EE组),运动预处理组(EP组),EP组采用持续4周的中等游泳负荷训练后,EE组和EP组负自身体重的3%进行一次性力竭运动游泳,用免疫组织化学法及免疫印迹法检测大鼠大脑GFAP的表达。结果:EE组GFAP表达强度较C组无显著性差异(P>0.05),EP组较EE组及C组显著增强(P<0.05);EP组大鼠力竭游泳时间较EE组明显延长(P<0.05)。结论:一次性力竭运动大脑中GFAP未见明显活化,可能参与了中枢性运动疲劳的病理生理过程,而运动预处理后GFAP可显著活化,这可能是其提高运动能力,缓解中枢性运动疲劳的机制之一。
OBJECTIVE: To establish a model of exercise preconditioning and a one-time exhaustive exercise model in SD rats, and to explore the effect of exercise preconditioning on the expression of glial acidity in cerebral cortex induced by one-time exhaustive exercise. Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups: control group (C), exhaustive exercise group (EE), exercise preconditioning group (EP). EP group received moderate swimming load After training, the EE group and the EP group were subjected to a one-time exhaustive swimming exercise at a negative body weight of 3%, and the expression of GFAP was detected by immunohistochemistry and immunoblotting. Results: The GFAP expression intensity in EE group was not significantly different from that in C group (P> 0.05), and EP group was significantly higher than that in EE group and C group (P <0.05). Exhaustive swimming time in EP group was longer than that in EE group P <0.05). Conclusion: There is no significant activation of GFAP in the brain of one-time exhaustive exercise, which may be involved in the pathophysiological process of central fatigue. GFAP may be activated significantly after exercise preconditioning, which may be its ability to improve exercise capacity and alleviate central fatigue One of the mechanisms.