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Trapping force of an optical tweezers system with an oil immersion objective is calculated with a ray-optics model.Results indicate that the trapping force will be decreased as a result of the introduction of spherical aberration,which is caused by the refractive mismatch between objective oil and water,when the sample manipulated is suspended in a water solution.The effect of spherical aberration will be serious when the detection depth of the optical tweezers is enhanced.