论文部分内容阅读
本文应用Abbott公司第三代酶免疫试验（EIA－3），与Abbott公司和国产第二代酶免疫试验（EM－2），对比检测了96例肝炎病人和献血员标本的抗-HCV，发现Abbott公司和国产EIA－2的相对漏检率分别为17．3％和20．7％，而献血员中有漏检10．8％。对部分漏检标本应用多聚酶链试验（PCR）检测了HCV－RNA，发现与HCV感染有关。这些是造成某些临床误诊及输血后肝炎的重要原因之一。
In this paper, Abbott’s third-generation enzyme immunoassay (EIA-3) was used to detect anti-HCV in 96 hepatitis patients and donors compared with Abbott and the second-generation domestic enzyme immunoassay (EM-2) The relative omission rates between Abbott and domestic EIA-2 were 17.3% and 20.7% respectively, while the undetected percentage of blood donors was 10.8%. HCV-RNA was detected by polymerase chain reaction (PCR) on part of missed samples and found to be associated with HCV infection. These are some of the major causes of misdiagnosis and post-transfusion hepatitis.