为研究端粒酶催化蛋白亚单位(caltalytic protein subunits of telomerase,hTERT)mRNA在肿瘤组织中的表达情况,建立一种恶性肿瘤诊断的新方法,通过提取肿瘤组织总RNA,用反转录聚合酶链式反应(RT-PCR)法对76例各型肿瘤组织及5例恶性胞腹水进行了检测。结果表明,76例各型肿瘤组织中66例阳性,5例恶性胸腹水全部阳性,表明RT-PCR法检测hTERTmRNA是一种灵敏、简便的恶性肿瘤诊断的新方法。 In order to study the expression of telomerase catalytic subunit of telomerase (hTERT) mRNA in tumor tissue, a new method for the diagnosis of malignant tumor was established. Total RNA was extracted from the tumor tissue by reverse transcription polymerase 76 cases of various types of tumor tissues and 5 cases of malignant ascites were detected by the method of chain reaction (RT-PCR). The results showed that in 76 cases of various types of tumor tissue, 66 cases were positive, and 5 cases of malignant pleural effusion were all positive, indicating that hTERT mRNA detection by RT-PCR is a sensitive and simple method for the diagnosis of malignant tumors.