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目的：研究rasp21、c-erbB-2、c-erbB-3、p53、nm23蛋白与细支气管肺泡癌（BAC）淋巴结转移的关系。方法：应用免疫组化LSAB法检测50例BAC（其中18例伴淋巴结转移）标本。结果：rasP21、c－erbB－2、c－erbB3、P53蛋白在伴或不伴淋巴结转移的BAC中的表达差异无显著性（P＞0．05）。但在18例伴淋巴结转移的BAC原发灶内，rasP21、c－erbB-2、c－erbB-3、p53中两种及两种以上同时表达的有4例（22％），而在相应的淋巴结转移灶中则为8例（44％）。nm23／NDPK在BAC中的表达（34／50，78％）高于癌旁肺实质（P＜0．01）。在伴淋巴结转移的BAC中nm23／NDPK表达率为56％（10／18），在无淋巴结转移的BAC中为91％（29／32），差别有高度显著性（P＜0．01）O结论：肿瘤转移受多种基因及其表达产物的调控。nm23基因的表达不仅与BAC的发生有关，而且在BAC中起到转移抑制基因的作用。
Objective: To investigate the relationship between rasp21, c-erbB-2, c-erbB-3, p53 and nm23 proteins and lymph node metastasis in bronchioloalveolar carcinoma (BAC). Methods: Immunohistochemical LSAB method was used to detect 50 specimens of BAC (18 of them with lymph node metastasis). RESULTS: The expression of rasP21, c-erbB-2, c-erbB3, P53 protein in BAC with or without lymph node metastasis showed no significant difference (P>0.05). However, in 18 primary BACs with lymph node metastasis, rasP21, c-erbB-2, c-erbB-3, and p53 were expressed in 4 and 22 (22%) at the same time. Of the lymph node metastases, 8 cases (44%). The expression of nm23/NDPK in the BAC (34/50, 78%) was higher than that in the paracarcinoma (P<0.01). The expression of nm23/NDPK was 56% (10/18) in BAC with lymph node metastasis, and 91% (29/32) in BAC without lymph node metastasis, and the difference was highly significant (P<0.01). Conclusion: Tumor metastasis is regulated by a variety of genes and their expression products. The expression of nm23 gene is not only related to the occurrence of BAC, but also plays a role of metastasis suppressor gene in BAC.