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目的:探讨细胞因子IL-21、SIL-2R在异基因造血干细胞移植(allo-HSCT)后急性移植物抗宿主病(aGVHD)发病机理中的作用。方法:观察20例Allo-HSCT患者aGVHD的发病情况,移植前后定期采集20例患者的外周血,采用双夹心酶联免疫吸附法(ELISA)检测其细胞因子IL-21、SIL-2R的浓度。结果:1.异基因造血干细胞移植后20例患者全部获得造血功能重建,中性粒细胞恢复到0.5×109/L及血小板恢复到20×109/L的中位时间分别为移植后13.5天及18天。2.发生aGVHD的患者,IL-21、SIL-2R浓度较移植前明显升高,IL-21、SIL-2R浓度在aGVHD阳性组明显高于aGVHD阴性组(P<0.01)。结论:1.细胞因子IL-21、SIL-2R在aGVHD的发病中起重要的正向调节作用,检测异基因造血干细胞移植后患者血清的IL-21、SIL-2R水平有助于预测aGVHD的发生。2.IL-21、SIL-2R与感染无相关性。
Objective: To investigate the role of cytokines IL-21 and SIL-2R in the pathogenesis of acute graft-versus-host disease (aGVHD) following allogeneic hematopoietic stem cell transplantation (allo-HSCT). Methods: The incidence of aGVHD in 20 patients with Allo-HSCT was observed. Peripheral blood samples of 20 patients were collected regularly before and after transplantation. The levels of IL-21 and SIL-2R were measured by double-sandwich enzyme-linked immunosorbent assay (ELISA). All of the 20 patients after allogeneic hematopoietic stem cell transplantation had hematopoietic reconstitution. The median time of neutrophil recovery to 0.5 × 109 / L and platelet recovery to 20 × 109 / L were 13.5 days after transplantation and 18 days. The levels of IL-21 and SIL-2R in patients with aGVHD were significantly higher than those before transplantation. The concentrations of IL-21 and SIL-2R in aGVHD-positive group were significantly higher than those in aGVHD-negative group (P <0.01). IL-21 and SIL-2R play an important role in the pathogenesis of aGVHD. The detection of IL-21 and SIL-2R levels in patients with allogeneic hematopoietic stem cell transplantation is helpful to predict aGVHD occur. 2.IL-21, SIL-2R no correlation with infection.