论文部分内容阅读
目的:评价死后细菌学检测在死因鉴定中的作用。方法:取健康SD大鼠30只,10只采用颈椎脱臼法处死纳入A组,10只制作缺氧缺血模型20min确认死亡纳入B组,10只注射金黄色葡萄球菌纳入C组,不给于治疗,待死亡,分别在死后3、6、9日,取后肢深部肌肉,采用化学发光法检测ATP水平,另取30只SD大鼠,同法处死,分别在死后第1-10日,采样取后肢深部肌肉、肝脏、脾脏、肾脏组织,进行细菌学培养镜检。结果:以ATP浓度对数值作为横坐标,以相对发光单位的对数值作为横坐标,呈一元线性方程Y=0.815X+9.015,R2=0.998,P<0.01;A组、B组、C组死后第3日、6日、9日ATP组间与组内比较差异具有统计学意义(P<0.05);普通培养、厌氧培养,A组、B组、C组肌肉、肝脏、脾脏、肾脏细菌培养阳性出现时间、优势菌类型差存在差异。结论:死后细菌学检测可用于死因鉴定中,ATP、细菌培养与鉴定等都可作为死因判断依据,但检测结果受观察时间等因素影响,同时是否适用于野外大型动物死亡尚无明确定论。
PURPOSE: To evaluate the role of post-mortem bacteriological tests in the identification of the cause of death. Methods: Thirty healthy SD rats were randomly divided into group A (10 rats), group A (10 rats), group B (10 rats), group B (10 animals), and group A Treatment, to be dead, respectively, on the 3rd, 6th and 9th days after death, deep muscle of the hind limb was taken. The level of ATP was measured by chemiluminescence method. Another 30 SD rats were sacrificed and killed in the same way Samples taken deep hindlimb muscle, liver, spleen, kidney tissue, bacteriological culture microscopy. Results: Using the logarithm of ATP concentration as the abscissa and the logarithm of the relative luminescence unit as the abscissa, the linear equation was Y = 0.815X + 9.015, R2 = 0.998, P <0.01. The A group, the B group and the C group died There was significant difference between the ATP group and the control group on the 3rd, 6th and 9th day (P <0.05). Normal, anaerobic, group A, B, C muscle, liver, spleen and kidney Bacterial culture appeared positive time, there are differences in the dominant type of bacteria. Conclusion: Bacteriological tests can be used to identify the causes of death after death. ATP, bacterial culture and identification can all be used as the basis for determining the cause of death. However, the test results are affected by the time of observation and other factors.