目的:探讨重组人脑利钠肽(rhBNP)对创伤失血性休克导致急性肺损伤(ALI)大鼠的肺血管通透性的影响。方法:60只SD大鼠随机分为6组:假手术组、模型组、生理盐水组、rhBNP治疗1h组、rhBNP治疗12h组、rhBNP治疗24h组,每组10只大鼠。假手术组完成所有手术操作,但不放血和复苏;模型组完成所有手术操作,放血后给予复苏;生理盐水组在实验前经尾静脉注射无菌生理盐水(2.5ml/kg);rhBNP治疗1、12、24h组大鼠在实验前经尾静脉注射rhBNP(30μg/kg)治疗。各组于术后6h处死大鼠,采集标本,进行肺组织病理学检测及评分,计算肺组织湿/干(W/D)比重、肺水含量,测定肺泡灌洗液(BALF)蛋白含量、髓过氧化物酶(MPO)活性和肺组织匀浆伊文思蓝(EB)含量,并采用酶联免疫吸附测定法(ELISA)测定血清中IL-6和TNF-α水平。结果:肺组织病理学结果显示rhBNP治疗组的肺损伤程度轻于模型组,且rhBNP治疗1、12、24h组肺损伤病理学评分均低于模型组,其中rhBNP治疗12h组和rhBNP治疗24h组评分与模型组比较,差异有统计学意义(P<0.05);rhBNP治疗12h组和rhBNP治疗24h组的BALF蛋白含量、肺W/D比值和肺水含量均明显低于模型组(P<0.05,P<0.01),且rhBNP治疗1、12、24h组的肺匀浆EB含量也明显低于模型组(P<0.05,P<0.01);rhBNP治疗1、12、24h组的肺匀浆MPO活力和血清IL-6、TNF-α水平明显低于模型组(P<0.05,P<0.01)。结论:rhBNP对创伤失血性休克导致的ALI有保护作用,可能是通过降低ALI时肺血管通透性来实现的。 Objective: To investigate the effect of recombinant human brain natriuretic peptide (rhBNP) on pulmonary vascular permeability in rats with acute lung injury (ALI) induced by traumatic hemorrhagic shock. Methods: Sixty Sprague-Dawley rats were randomly divided into 6 groups: sham operation group, model group, saline group, rhBNP for 1 h group, rhBNP for 12 h group and rhBNP for 24 h group, with 10 rats in each group. Sham operation group completed all the operation, but did not give up blood and resuscitation; model group completed all the operation operation, give blood recovery after resuscitation; saline group before experiment through the tail vein injection of sterile saline (2.5ml / kg); rhBNP treatment 1 The rats in 12 and 24 hours group were treated with rhBNP (30μg / kg) by tail vein injection before experiment. The rats were sacrificed at 6h after operation, and the specimens were collected for lung histopathological examination and score. The wet / dry (W / D) specific gravity and pulmonary water content of lung tissue were calculated. The contents of alveolar lavage fluid (BALF) Myeloperoxidase (MPO) activity and Evans blue (EB) content in lung homogenates were measured. Serum levels of IL-6 and TNF-α were measured by enzyme linked immunosorbent assay (ELISA). Results: The results of lung histopathology showed that the degree of lung injury in rhBNP-treated group was lighter than that in model group, and the pathological scores of lung injury in rhBNP group 1, 12 and 24 h were lower than those in model group, including rhBNP 12 h and rhBNP 24 h (P <0.05). Compared with the model group, the BALF protein content, lung W / D ratio and lung water content in rhBNP group and rhBNP group for 24h were significantly lower than those in model group (P <0.05) , P <0.01). The content of EB in lung homogenate of 1, 12 and 24 hours rhBNP treatment group was also significantly lower than that of model group (P <0.05, P <0.01) Vigor and serum levels of IL-6 and TNF-α in the model group were significantly lower than those in the model group (P <0.05, P <0.01). Conclusion: rhBNP has a protective effect on ALI induced by traumatic hemorrhagic shock, which may be achieved by decreasing pulmonary vascular permeability during ALI.