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介绍了血和尿两种生物体液中吗啡的固相提取方法。采用衍生化气相色谱法作为测试手段。系统考查了５种国产和进口固相萃取剂、３种洗脱剂及提取ｐＨ值对尿和血中吗啡提取率的影响。实验结果表明：国产ＧＤＸ－３０１、ＧＤＸ－４０３和进口Ｃ１８等固相萃取剂均对吗啡有较好的提取行为；氯仿：异丙醇（９：１）对固相萃取剂上的吗啡有较强的洗脱行为；若采用ＧＤＸ－３０１为固相萃取剂，尿中吗啡在ｐＨ值为９左右时，提取率可达９０％以上，而后提取率随ｐＨ值提高下降；血中吗啡随ｐＨ值提高，提取率提高，在强碱状态下，提取率可达９０％以上
The solid phase extraction method of morphine in blood and urine is introduced. Derivatization gas chromatography is used as a test method. The effects of five kinds of domestic and imported SPE, three kinds of eluents and extraction of pH on the extraction rate of morphine in urine and blood were systematically investigated. The experimental results showed that solid-state extraction of domestic GDX-301, GDX-403 and imported C18 solid phase extraction solvent had better extraction behavior; chloroform: isopropanol (9: 1) Strong elution behavior; if using GDX-301 as solid phase extraction solvent, morphine in urine at pH value of about 9, the extraction rate of up to 90%, and then the extraction rate increased with the increase of pH; blood morphine with pH The value increased, the extraction rate increased in the strong alkali state, the extraction rate of up to 90%