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目的:探讨参附注射液对心肺复苏后大鼠肺组织核转录因子κB(NF-κB)及血浆肿瘤坏死因子α(TNF-α)的影响。方法:90只Sprague Dawley大鼠随机分为假手术组、模型组、参附治疗组,采用窒息合并冰氯化钾致大鼠心跳骤停-心肺复苏模型,运用免疫组化方法观察复苏后肺组织细胞中NF-κB的蛋白表达,采用放射免疫法检测血浆TNF-α水平;通过光镜和电镜观察肺组织细胞结构的变化。结果:与假手术组比较,复苏后肺组织NF-κB表达及血浆TNF-α水平均明显增加(P<0.01),NF-κB及血浆TNF-α分别于复苏后12 h和24 h达到高峰,并持续到48 h。与模型组相比,参附治疗组各时相点NF-κB及TNF-α表达均明显下降(P<0.05)。光镜和电镜观察结果显示参附治疗组肺组织细胞结构受损明显减轻。结论:参附注射液可能通过抑制NF-κB活性及降低TNF-α水平,从而阻断NF-κB调控的炎症反应及细胞凋亡,对复苏后肺组织损伤起到防治作用。
Objective: To investigate the effect of Shenfu injection on nuclear factor κB (NF-κB) and plasma tumor necrosis factor-α (TNF-α) in rat lung after cardiopulmonary resuscitation. Methods: 90 Sprague Dawley rats were randomly divided into sham-operation group, model group and Shenfu treatment group. The rats were subjected to CPB-CPR model by asphyxia combined with ice-cold potassium chloride (KP) The expression of NF-κB protein in tissue cells was detected by radioimmunoassay. Plasma TNF-αlevel was detected by light microscope and electron microscope. Results: Compared with the sham operation group, the expression of NF-κB in lung tissue and the level of TNF-α in plasma increased significantly after resuscitation (P <0.01), while the levels of NF-κB and TNF-α peaked at 12 h and 24 h after resuscitation , And continued to 48 h. Compared with the model group, the expression of NF-κB and TNF-α in Shenfu treatment group were significantly decreased at each time point (P <0.05). Light and electron microscopy results show that the treatment group significantly reduced lung tissue damage. Conclusion: Shenfu injection may prevent NF-κB-mediated inflammation and apoptosis by inhibiting the activity of NF-κB and decreasing the level of TNF-α, which may prevent lung injury after resuscitation.