论文部分内容阅读
目的比较筛选最适于贝母属药用贝母的总DNA提取方法。方法利用试剂盒法、改良的CTAB法、改良的SDS法提取贝母属药用贝母的总DNA;通过核酸蛋白检测仪及琼脂糖凝胶电泳方法检测其浓度和纯度;进行ISSR-PCR扩增检测所得总DNA质量。结果 3种方法均可提取出产量较高的基因组DNA,但试剂盒法相较提取的总DNA纯度最高,质量最好,并且均能扩增出丰富清晰的条带,稳定性高,操作简单耗时短。改良的CTAB法、SDS法提取的总DNA纯度、质量均次于试剂盒法,操作复杂耗时长,不适用于下游的分子生物学实验。结论试剂盒法为贝母属药用贝母总DNA提取的最佳方法,其所得样品适用于PCR及其他分子生物学研究。
Objective To compare and screen the most suitable DNA extraction method for Fritillaria cirrhosa. Methods The total DNA of Fritillaria cirrhosa was extracted by kit method, modified CTAB method and modified SDS method. The concentration and purity of Fritillaria cirrhosa were determined by nucleic acid protein detector and agarose gel electrophoresis. The ISSR-PCR Increase the total DNA quality test. Results Genomic DNA with high yield could be extracted by all three methods. However, the total DNA extracted by the kit method had the highest purity and best quality, and all of them could amplify rich and clear bands with high stability and simple operation Short time. The modified CTAB method and SDS method are both inferior to kit method in the purity and quality of total DNA. The operation is complicated and time-consuming, which is not suitable for downstream molecular biology experiments. Conclusion The kit method is the best way to extract the total DNA of Fritillaria cirrhosa. The obtained sample is suitable for PCR and other molecular biology research.