论文部分内容阅读
目的:应用抑制性消减杂交(SSH)技术构建乙型肝炎病毒(HBV)X蛋白(HBxAg)反式激活基因XTP3的差异表达的cDNA消减文库,克隆XTP3反式激活相关基因。方法:依据我室构建的HBV X蛋白反式激活基因XTP3差异表达的cDNA消减文库,利用生物信息学技术获得新基因XTP3TPA的编码序列,对其可能的氨基酸序列进行分析比较,并对其进行克隆化研究。结果:发现了HBV XTP3反式激活作用的新的靶基因,命名为XTP3TPA。结论:这一发现为阐明HBV XTP3蛋白的反式激活作用及其机制开辟了新的研究方向。
OBJECTIVE: To construct a cDNA subtractive library of differentially expressed XTP3 gene of hepatitis B virus (HBV) X protein (HBxAg) by suppression subtractive hybridization (SSH) and clone the transactivation related gene of XTP3. Methods: Based on the subtractive cDNA library of XTP3, a transactivator of HBV X protein, constructed in our laboratory, the coding sequence of novel gene XTP3TPA was obtained by using bioinformatics technology. The possible amino acid sequences were analyzed and compared and cloned Research. Results: A new target gene of HBV XTP3 transactivation was found named XTP3TPA. Conclusion: This finding opens up new research directions for clarifying the transactivation and mechanism of HBV XTP3 protein.