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目的探讨血管生成与骨肉瘤临床病理及预后的关系。方法通过内皮细胞特异性标记物ＦⅧ－ＲＡｇ和ＣＤ３１免疫组化染色，计数７０例骨肉瘤微血管密度。结果肿瘤微血管密度与骨肉瘤体积，Ｐｒｉｃｅ′ｓ分级和Ｄａｈｌｉｎ分型无关，而与肿瘤性骨样组织分型，ＷＨＯ新分型及ＰＣＮＡ标记指数有关。Ｋａｐｌａｎ－Ｍｅｉｅｒ分析微血管计数＜３１（×２００）的骨肉瘤预后较好，而＞３１（×２００）的骨肉瘤预后较差。结论结果表明骨肉瘤微血管密度与生存率相关，提示肿瘤血管生成分析可能是骨肉瘤一项有价值的预后指标。
Objective To investigate the relationship between angiogenesis and clinical pathology and prognosis of osteosarcoma. Methods Immunohistochemical staining of endothelial cell specific markers FVIII-RAg and CD31 was performed to count the microvessel density of osteosarcoma in 70 cases. Results The tumor microvessel density was not related to osteosarcoma volume, Price’s grade and Dahlin classification, but related to neoplastic osteoid tissue type, WHO new typing and PCNA labeling index. Kaplan-Meier analysis showed that the prognosis of osteosarcoma with microvessel count <31 (×200) was better, while the prognosis of osteosarcoma with >31 (×200) was poor. Conclusions The results indicate that the microvessel density and survival rate of osteosarcoma are related, suggesting that tumor angiogenesis analysis may be a valuable prognostic indicator of osteosarcoma.