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目的研究嗜酸乳杆菌ＤＯＭＬａ菌株是否对致泻性大肠埃希菌对上皮细胞粘附和侵袭能力具有抑制作用。方法使用了Ｈｅｐ－２细胞粘附实验和Ｈｅｐ－２细胞侵袭实验。结果发现嗜酸乳杆菌ＤＯＭＬａ菌株对Ｈｅｐ－２细胞粘附平均达２２２．４８±３０．７个细菌／细胞。ｔｈｙＡ基因突变菌株ＤＯＭＬａＦ８４对Ｈｅｐ－２细胞的粘附为６１．９６±１０．２７个细菌／细胞。将嗜酸乳杆菌ＤＯＭＬａ菌株与肠产志贺氏样毒素且具有侵袭力的大肠埃希氏菌（ＥＳＩＥＣ）Ｆ１７１菌株共同孵育后，可将后者的粘附和侵袭菌数分别由２７３．４±２４．７个细菌／细胞和１３．４±４．９个细菌／细胞，降低到６５．３±１３．１个细菌／细胞和６．８２±１．６个细菌／细胞，差异有显著性（Ｐ＜０．０１）。将ＤＯＭＬａ菌株与肠侵袭性大肠埃希氏菌（ＥＩＥＣ）８４０１菌株共同孵育后，可将后者对Ｈｅｐ－２细胞的粘附和侵袭数分别由２６９．２±２６．５个细菌／细胞和３４．０±５．３个细菌／细胞降到７０．７±１４．０个细菌／细胞和６．８２±２．９个细菌／细胞，差异有显著性（Ｐ＜０．０１）。结论嗜酸乳杆菌ＤＯＭＬａ菌株对ＥＳＩＥＣＦ１７１菌株和ＥＩＥＣ８４０１菌株的Ｈｅｐ－２细胞的粘附和侵袭能力?
Objective To study whether Lactobacillus acidophilus DOMLa could inhibit the ability of diarrheal Escherichia coli to adhere to and invade epithelial cells. Methods Hep-2 cell adhesion assays and Hep-2 cell invasion assays were used. As a result, it was found that the Lactobacillus acidophilus DOMLa strain had an average of 222.48 ± 30.7 bacteria / cell on Hep-2 cell adhesion. The adhesion of thyA gene mutant strain DOMLaF84 to Hep-2 cells was 61.96 ± 10.27 bacteria / cell. After co-incubation of the L. acidophilus DOMLa strain with the enteric-producing shiga-like toxin-producing Escherichia coli (ESIEC) F171 strain, the number of adhesion and invasion of the latter strain increased from 273.4 ± 24.7 bacteria / cell and 13.4 ± 4.9 bacteria / cell, down to 65.3 ± 13.1 bacteria / cell and 6.82 ± 1.6 bacteria / cell, the difference was significant (P <0.01). Incubation of the DOMLa strain with the E. coli 8401 strain of enterotoxigenic Escherichia coli (EIEC) showed that the adhesion and invasion rates of the latter to Hep-2 cells were increased from 269.2 ± 26.5 bacteria / cell and 34.0 ± 5.3 bacteria / cell down to 70.7 ± 14.0 bacteria / cell and 6.82 ± 2.9 bacteria / cell, the difference was significant (P <0.01). Conclusion The ability of Lactobacillus acidophilus DOMLa to adhere to and invade Hep-2 cells of ESIECF171 and EIEC8401 strains?