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目的观察三种常用化学消毒剂对胃镜污染的乙肝病毒消毒效果。方法采用PCR检测方法,对酸性氧化电位水、邻苯二甲醛和戊二醛等三种化学消毒剂消除胃镜中残留乙肝病毒的效果进行观察。结果污染HBVDNA阳性血清的胃镜,仅经过规范清洗程序,即可有效清除镜体表面污染的HBV-DNA达99%以上,可有效清除内腔污染的HBV-DNA达99.99%以上。在常规清洗的基础上,酸性氧化电位水和5 500 mg/L邻苯二甲醛浸泡5 min,对污染在胃镜表面的HBV-DNA灭活率为91%以上,对胃镜内腔HBV-DNA灭活率为100%;戊二醛浸泡10 min,对胃镜表面HBV-DNA灭活率为86.76%,对胃镜内腔HBV-DNA灭活率为100%。结论胃镜清洗质量决定了消毒效果;在污染胃镜的清洗过程中,外表和内腔清洗同样不容忽视。
Objective To observe the disinfection effect of three common chemical disinfectants on gastroscope-contaminated hepatitis B virus. Methods The effect of three kinds of chemical disinfectants such as acid oxidation potential water, phthalaldehyde and glutaraldehyde on eliminating residual hepatitis B virus in gastroscope was observed by PCR method. Results Endoscopy of HBVDNA-positive sera contaminated HBV-DNA of only 99% of the surface of the mirror can effectively remove the contamination of HBV-DNA in the luminal cavity by 99.99% or more only after standard cleaning procedures. On the basis of routine cleaning, the acid oxidation potential water and 5 500 mg / L o-phthalic aldehyde soaked for 5 min, the inactivation rate of HBV-DNA on gastroscope surface was over 91% The survival rate was 100%. After soaking with glutaraldehyde for 10 min, the inactivation rate of HBV-DNA on gastroscopic surface was 86.76% and the inactivation rate of HBV-DNA in endoscopic cavity was 100%. Conclusion The quality of gastroscope cleaning determines the disinfection effect. During the cleaning of gastroscope, the appearance and internal cavity cleaning should not be ignored.