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A sensitive,specific and rapid LC-MS method was developed and validated for the determination of salvianolic acid D(Sal D) in rat plasma.This method used a single quadrupole mass spectrometer with an electrospray ionization(ESI) source.A single ion monitoring scanning(SIM) mode was employed.It showed good linearity over the concentration range from 3.3 to 666.7 ng/m L for the determination of Sal D.The R.S.D.% of intra-day and inter-day precision values were no more than7.69%,and the accuracy was within 91% 104% at all quality control levels.This LC-MS method was applied to the pharmacokinetic study of Sal D in rats.A two-compartmental model analysis was employed.The plasma concentrations at 2 min(C2min) were 5756.067719.61,11,073.0171783.46 and21,077.5875581.97 μg/L for 0.25,0.5 and 1 mg/kg intravenous injection,respectively.The peak plasma concentration(Cmax) was 333.08761.21 μg/L for 4 mg/kg oral administration.The area under curve(AUC0 t) was 14,384.37978443.184,22,813.369711,860.823,46,406.122727,592.645 and8201.74074711.961 μg/L h for intravenous injection(0.25,0.5 and 1 mg/kg) and oral administration(4 mg/kg),respectively.The bioavailability of Sal D was calculated to be 4.159%70.517%.
A sensitive, specific and rapid LC-MS method was developed and validated for the determination of salvianolic acid D (Sal D) in rat plasma. This method used a single quadrupole mass spectrometer with an electrospray ionization (ESI) source. A single ion monitoring scanning (SIM) mode was employed. It showed good linearity over the concentration range from 3.3 to 666.7 ng / m L for the determination of Sal D. RSD% of intra-day and inter-day precision values were no more than 7.69 %, and the accuracy was within 91% 104% at all quality control levels. This LC-MS method was applied to the pharmacokinetic study of Sal D in rats. A two-compartmental model analysis was employed. Plasma concentrations at 2 min ( C2min) were 5756.067719.61, 11,073.0171783.46 and 21,077.5875581.97 μg / L for 0.25,0.5 and 1 mg / kg intravenous injection, respectively.The peak plasma concentration (Cmax) was 333.08761.21 μg / L for 4 mg / kg oral administration. The area under curve (AUC0 t) was 14,384.37978443.184,22,813.369711,860 .823,46,406.122727,592.645 and8201.74074711.961 μg / L for intravenous injection (0.25,0.5 and 1 mg / kg) and oral administration (4 mg / kg), respectively.The bioavailability of Sal D was calculated to be 4.159% 70.517 %.