论文部分内容阅读
目的 研究 型胶原、层粘连蛋白 (lam inin,L N)和间质金属蛋白酶 - 2 (matrix m etalloproteinase- 2 ,MMP- 2 )在不同年龄小鼠卵巢中的分布和变化模式。 方法 利用生物素 -抗生物素蛋白间接免疫荧光方法 ,从蛋白水平观察 型胶原和 L N的分布和变化 ;利用地高辛标记的 型胶原和 MMP- 2基因探针进行原位杂交 ,从m RNA水平观察 型胶原和 MMP- 2的分布和变化。 结果 出生 5 d小鼠卵巢的卵泡基膜和卵巢表面上皮(ovarian surface epithelium,OSE)基膜处已有少量 型胶原和 L N分布 ,基质细胞和 OSE中也有分布。 型胶原和 MMP- 2的 m RNA在基质细胞和 OSE有表达。发育期小鼠卵巢中卵泡基膜和 OSE基膜中 型胶原和 L N的含量随年龄的增加而呈增加趋势 ,分布也趋于连续。颗粒细胞、膜细胞、OSE中都有 型胶原和 L N分布 ,也有 型胶原和 MMP- 2 m RNA的表达 ,其表达水平明显比 5 d时高。成熟期小鼠卵巢 型胶原和 L N的含量较高。12个月的小鼠卵巢已开始退化 ,基膜细胞外间质 (extracellular matrix,ECM)的数量减少 ,MMP- 2 m RNA的表达量无明显变化。 结论 型胶原和 L N在小鼠卵巢发育过程中含量和分布发生着动态变化 ,可能对卵泡发育起重要作用
Objective To study the distribution and changes of lam inin (L N) and matrix metalloproteinase-2 (MMP-2) in the ovaries of mice of different ages. Methods The distribution and changes of collagen and LN were observed by biotin-avidin indirect immunofluorescence method. In situ hybridization was performed using digoxigenin-labeled collagen and MMP-2 gene probe, Horizontal observation of collagen and MMP-2 distribution and changes. Results Small amounts of collagen and LN were found in the basement membrane of ovarian follicles and ovarian surface epithelium (OSE) on the 5th day after birth, and were also distributed in stromal cells and OSE. Collagen and MMP-2 mRNA are expressed in stromal cells and OSE. The content of middle collagen and L N in the ovarian follicle basement membrane and OSE basement membrane of mice during the developmental period increased with the increase of age, and the distribution also tended to be continuous. There were type collagen and L N distribution in granulosa cells, membranous cells and OSE, as well as the expression of type I collagen and MMP-2 m RNA. The expression level was significantly higher than that at 5 d. Mature mouse ovary collagen and L N content is higher. The ovaries of 12-month-old mice have begun to degenerate, the number of extracellular matrix (ECM) in the basement membrane has decreased, and the expression of MMP-2 mRNA has not changed significantly. Conclusion The content and distribution of type I collagen and L N in mouse ovary development have a dynamic change and may play an important role in follicular development