论文部分内容阅读
本研究以 3个高抗粗缩病的玉米自交系 (P138、齐 319、H2 1)和 3个感病玉米自交系 (478、 10 7、Mo17)以及杂交组合 P138× 4 78为材料 ,寻找与玉米粗缩病 (MRDV)基因紧密连锁的分子标记 ,并对其辅助选择效果进行了初步探讨。结果表明 :引物 S37所扩增的多态性片段 S37+ 2 0 0 0 和 S86所扩增的多态性片段 S86 - 1 30 0 与 MRDV抗性基因相连锁 ,S37+ 2 0 0 0 和 S86 - 1 30 0 分别是该基因的正负 RAPD标记带。利用正标记带 S37+ 2 0 0 0 和负标记带 S86 - 1 30 0 对 F2 群体和 F3株系连续进行两个世代的选择 ,选择效果明显。正标记随世代增加抗病率上升 ,F2 、 F3、 F4 群体的抗病株率分别为 87.75 %、91.2 0 %和 94 .79% ;负标记随世代增加抗病率下降 ,F2 、 F3、 F4 群体的抗病株率分别为 87.75 %、 85 .32 %和 75 .39%。
In this study, three inbred lines (P138, Qi 319, H2 1) and three susceptible maize inbred lines (478, 107, Mo17) and P138 × 4 78 , Looking for molecular markers closely linked to the maize rough-cut disease (MRDV) gene, and initially explored its adjuvant selection effect. The results showed that the polymorphic fragment S86 + 1203 amplified by S37 and S86-130 0 amplified by S86 was linked with MRDV resistance gene, S37 + 20000 and S86-1 30 0, respectively, the positive and negative RAPD marker gene band. The F2 population and the F3 strain were continuously selected for two generations with positive marker S37 + 20000 and negative marker S86-130 0, and the selection effect was obvious. Positive markers increased with disease resistance rate of generations, F2, F3, F4 population of resistant strains were 87.75%, 91.2 0% and 94. 79%; negative markers decreased with increasing generation resistance, F2, F3, F4 The resistant rate of the population was 87.75%, 85.32% and 75.39% respectively.