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以迟钝爱德华氏菌的代表菌株(HC010907-1)为免疫原,制备免疫血清,对分离于牙鲆鱼的130株迟钝爱德华氏菌进行了血清型检定,结果表明:供试的130株迟钝爱德华氏菌均为同种血清型;同时以此免疫血清为第一抗体,以标准羊抗兔IgG荧光抗体为第二抗体,进行了荧光抗体技术检验迟钝爱德华氏菌的可行性试验,表明亦具有较强的特异性。
Serotypes of 130 strains of Erwinia edyceps isolated from flounder were prepared by using the representative strain of D. duodenum (HC010907-1) as an immunogen. The results showed that 130 tested iris Bacteria are all the same type of serotypes; at the same time the immune serum as the first antibody, the standard goat anti-rabbit IgG fluorescent antibody as the second antibody, fluorescence antibody test to test the feasibility of retarding Edwardsiella, also have Strong specificity.