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目的研究体外过氧化氢对生育男性精子凋亡及精子DNA完整性的影响。方法58例正常生育男性均来自吉林大学第二临床医院泌尿男科,精子Percoll优选后制作精子悬液,用伊红Y染色分析精子活率,用瑞-姬染色分析精子凋亡率,用精子核吖啶橙荧光染色检测精子DNA完整性。结果外加过氧化氢浓度为0·02mmol/l时,精子活率显著低于对照组(P(0·05)。当浓度为0·20mmol/l时,精子活率显著低于对照组(P(0·05),精子凋亡率显著高于对照组(P(0·05)。外加过氧化氢浓度为0·02mmol/l、0·2mmol/l时,精子DNA完整性均显著低于对照组(P(0·05),且精子DNA完整性随实验浓度增加而下降。结论体外过氧化氢对精子活率、凋亡率与DNA完整性有影响,高浓度的过氧化氢可促使精子凋亡,损伤精子DNA完整性,导致男性不育。
Objective To study the effects of hydrogen peroxide on sperm apoptosis and sperm DNA integrity in fertile men. Methods 58 normal fertile men were all from Urology Department of Second Clinical Hospital of Jilin University. Spermatozoa were perfused with spermatozoa Percoll, sperm motility was analyzed by Eosin Y staining, sperm apoptosis rate was analyzed by Reiki - Ji staining, Acridine orange fluorescence detection of sperm DNA integrity. Results When the concentration of hydrogen peroxide was 0.02 mmol / l, the sperm motility was significantly lower than that of the control group (P <0.05). When the concentration was 0.20 mmol / l, the sperm motility was significantly lower than that of the control group (P (0.05), the apoptosis rate of sperm was significantly higher than that of the control group (P <0.05) .The DNA integrity of the sperm was significantly lower than that of the control when the concentration of hydrogen peroxide was 0.02 mmol / l and 0.2 mmol / l Control group (P <0.05), and the integrity of sperm DNA decreased with the increase of experimental concentration.Conclusion Hydrogen peroxide has effects on sperm motility, apoptosis rate and DNA integrity in vitro.High concentration of hydrogen peroxide can promote Sperm apoptosis, damage sperm DNA integrity, leading to male infertility.