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目的:观察金刚健骨片对去卵巢大鼠骨组织中TGF-β1及IGF-1表达的影响,进一步探讨金刚健骨片治疗绝经后骨质疏松症的作用机制。方法:将健康清洁级雌性SD大鼠40只随机分为5组,即假手术(A)组、金刚健骨片大剂量(B)组、金刚健骨片小剂量(C)组、雌激素(D)组、模型(E)组。所有大鼠常规饲料喂养,造模成功后使用对应药物灌胃治疗12周。治疗结束后处死大鼠采集第三腰椎为标本,采用免疫组化法检测TGF-β1及IGF-1在骨组织中的表达。数据使用SPSS16.0软件统计,检验水准α=0.05.结果:①TGF-β1在骨组织中的表达,B组、D组与E组比较差异有统计学意义(P<0.05);B组与D组比较其差异无统计学意义(P>0.05)。C组与E组比较其差异无统计学意义(P>0.05);C组、E组与A组比较其差异均有统计学意义(P<0.01)。②IGF-1各组间比较差异无统计学意义。结论:①金刚健骨片能使TGF-β1在骨组织中的表达增强,其治疗作用与雌激素治疗相仿,并能修复或者改善骨组织微细结构。②骨组织中IGF-1含量与雌激素关联不明显,金刚健骨片可略提高IGF-1的表达,但无统计学意义。
Objective: To observe the effect of Jingangjianjian Tablets on the expression of TGF-β1 and IGF-1 in the bone tissue of ovariectomized rats and to explore the mechanism of Jingangjian bone tablet in the treatment of postmenopausal osteoporosis. Methods: Forty healthy female Sprague-Dawley rats were randomly divided into five groups: sham operation (A), high dose of Jingangjian bone tablet (B), low dose of Jingangjian bone tablet (C) (D) group, model (E) group. All rats were fed with normal diet, and after successful modeling, the rats were treated with the corresponding drugs for 12 weeks. At the end of the treatment, the third lumbar vertebrae were collected and the expression of TGF-β1 and IGF-1 in bone was detected by immunohistochemistry. The data were analyzed by SPSS 16.0 software, the test level was α = 0.05.Results: ① The expression of TGF-β1 in bone tissue, there was significant difference between group B, D and E (P <0.05) There was no significant difference between the two groups (P> 0.05). There was no significant difference between group C and group E (P> 0.05). There was significant difference between group C and group E and group A (P <0.01). ② IGF-1 among the groups was no significant difference. Conclusion: ①JGG can enhance the expression of TGF-β1 in bone tissue and its therapeutic effect is similar to that of estrogen treatment, and can repair or improve the fine structure of bone tissue. ② The IGF-1 content in bone tissue was not significantly correlated with estrogen, but Jinggangjian bone tablet could slightly increase the expression of IGF-1, but there was no statistical significance.