目的 探讨电子束选区熔化(electron beam melting,EBM)和激光选区熔化(select laser melting,SLM)三维打印工艺对钛合金表面性能及细胞的影响,为三维打印钛合金的临床应用提供依据.方法 应用EBM和SLM制备钛合金(Ti-6A1-4V)试件,以锻造钛合金试件作为对照组.检测3组试件表面成分及EBM和SLM试件表面接触角、粗糙度及表面形貌.同时观察3组试件上骨髓间充质干细胞(bone marrow mesenchymal stem cell,BMSC)的黏附、增殖和细胞超微结构,并比较细胞培养液离子析出情况.结果 EBM和SLM试件表面接触角均＞65°,均为疏水性材料;两组试件表面粗糙度、表面形态相似,均可形成钛氧化膜,但不是利于细胞黏附的最佳表面结构.3组析出Ti、A1、V离子的含量均为微克级,不足以对细胞黏附和增殖造成影响.BMSC在3组试件上的黏附和增殖能力相近,随着培养时间延长,细胞数量增加明显;未见黏附于3组试件上的BMSC细胞器超微结构有损伤.结论 EBM与SLM工艺制备的钛合金具有良好的表面性能及细胞相容性.“,”Objective To evaluate the surface characteristics and cytocompatibility of Ti-6Al-4V alloy fabricated using select laser melting (SLM) and electron beam melting (EBM) technique.Methods Ti-6Al-4V alloy specimens were fabricated with SLM and EBM.A wrought form of Ti-6Al-4V alloy was used as a control.Its properties were evaluated using component analysis,contact angle test,surface roughness,surface topography,cell ultrastructure,cell attachment and proliferation observation,metal ion precipitation examination.Results The roughness of SLM and EBM specimens was suitable for cell attachment but not the best.The character of SLM and EBM specimens was hydrophobic (＞65°).The surface topography of EBM and SLM specimens were similar,but were not the best type for cell attachment.The components of Ti-alloy oxide film were detected in all the specimens.The content of Ti,A1,V ions of EBM,SLM and wrought specimens were very low and did not affect the cell attachment and proliferation.The ultrastructure of cell was normal,and the cytomembrane was intact.The number of cells was similar to each other among the three kinds of specimens and increased obviously with the culture time.Conclusions The results of the study suggested that EBM and SLM Ti-6A1-4V specimens possessed good surface characteristics.However,the surface modification are needed further.