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对棉花抗黄萎病品种(春矮早和86- 6)和感病品种(中棉17和豫棉12)的根际真菌区系进行了分析。结果表明每个品种都有自己独特的根际微生物区系,抗病品种根际微生物种数多于感病品种,区系组成更为复杂。抗病品种根际真菌优势种主要是漆斑菌 (Myrothecium )、粘帚霉(Gliocladium )、曲霉(Aspergillus)和青霉(Penicillium )等,感病品种则多为头孢霉(Cep halosporium )、镰刀菌(Fusarium )、矛束孢(Doratomyces)、粘帚霉(Gliocladium )和漆斑菌(Myrothecium )等。采用平板对峙培养法测定了不同抗性棉花品种根际真菌区系各成员对棉花黄萎病菌的抑制作用,发现抗病品种根际真菌区系中对黄萎病菌具有抑制作用的真菌种数和比率都高于感病品种,而且抗病品种根际真菌的优势种对棉花黄萎病菌的抑制作用较强。这种差异在苗期和现蕾期更为明显,花铃期则显著减小。研究结果表明棉花品种对棉花黄萎病的抗性与根际真菌区系组成及其对黄萎病菌的抑制能力有一定关系。
The rhizosphere fungal flora of resistant varieties of Verticillium dahliae (Chunqianzao and 86-6) and susceptible varieties (Zhongmian 17 and Yumian 12) were analyzed. The results showed that each species had its own unique rhizosphere microbial flora, and more rhizosphere microorganisms in susceptible varieties than susceptible varieties, and the floristic composition was more complicated. The dominant rhizosphere fungi of disease-resistant cultivars were Myrothecium, Gliocladium, Aspergillus and Penicillium. The susceptible cultivars were mostly Cep halosporium, Fusarium, Doratomyces, Gliocladium and Myrothecium, among others. The panel antagonistic culture method was used to determine the inhibitory effect of each member of rhizosphere fungal flora of different resistant cotton varieties on Verticillium dahliae. The results showed that the fungal species of Verticillium wilt The rates were higher than the susceptible varieties, and the dominant fungi of the resistant varieties of Rhizosphere Fungi had a stronger inhibitory effect on Verticillium dahliae. This difference is more obvious at seedling stage and budding stage, while the flower-boll stage is significantly reduced. The results showed that the resistance of cotton cultivars to Verticillium dahliae was related to the composition of rhizosphere fungi and their ability to inhibit Verticillium dahliae.