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目的 建立毛细管区带电泳 (Capillaryfreezoneelectrophoresis,CFZE)测定尿中反 ,反 粘康酸 (t,t MA)的方法 ,并评价其在苯接触工人健康监护中的应用价值。方法 应用CFZE方法 ,以“Z”型高灵敏检测器直接定性定量测定尿中t,t MA。并以此方法测定 1 5 5名苯作业工人 (其中 84人的接触水平为 0 .5～ 1 1 .2mg/m3 ,71人的接触水平为 6 .7～2 5 .6mg/m3 )和 5 1名管理人员 (对照组 )尿中t,t MA含量。结果 该法的平均回收率和相对标准差为 94 1 %和 5 1 5 %。最低检测限为 30 μg/L。接触组尿中t,t MA含量远高于对照组 [厂 1为 ( 1 .72± 1 .0 1 )mg/g肌酐、厂 2为 ( 4.87± 2 .34 )mg/g肌酐、对照组为 ( 0 .1 4± 0 .0 9)mg/g肌酐 ,P <0 .0 0 0 1 ]。接触组中 ,尿中t,t MA与空气中苯浓度呈现相关 (r =0 .86和r=0 .89,P <0 .0 0 0 1。在对照组中 ,吸烟者尿中t,t MA含量也较不吸烟者为高 [( 0 .1 8± 0 .1 4)mg/g肌酐与 ( 0 .1 2±0 0 3)mg/g肌酐 ,P =0 .0 2 ]。结论 以CFZE测定苯作业工人尿中t,t MA的方法具有简便、精密的特性 ,所得结果与文献报道数值相近 ,证明以本方法测定接触者尿中t,t MA可以反映苯的接触。
OBJECTIVE To establish a method for the determination of t, t-MA in urine by Capillary zone electrophoresis (CFZE) and to evaluate its value in the health monitoring of benzene exposure workers. Methods The CFZE method was used to qualitatively and quantitatively determine t, t MA in urine with “Z” type high sensitivity detector. And this method was used to determine the number of benzene workers (including 84 workers with a contact level of 0.5-1.2 mg / m3 and 71 persons with a contact level of 6.7-7.56 mg / m3) and 5 A management staff (control group) urinary t, t MA content. Results The average recovery and relative standard deviation of the method were 94 1% and 51 5%. The minimum detection limit is 30 μg / L. The content of t and t MA in the urine of the contact group was much higher than that of the control group [(1.72 ± 1.101) mg / g creatinine in factory 1 and (4.87 ± 2.34) mg / g creatinine in factory 2 and control group (0 .14 ± 0. 0 9) mg / g creatinine, P <0 0 0 1]. In the exposure group, urinary t, t MA correlated with benzene concentration in the air (r = 0.86 and r = 0.89, P0.001). In the control group, urinary t, The t MA content was also higher than the non-smokers [(0.18 ± 0.14) mg / g creatinine and (0.12 ± 0.333) mg / g creatinine, P = .02]. Conclusion The method of CFZE for the determination of t, t MA in urine of benzene workers is simple and precise. The obtained results are similar to those reported in literatures. It is proved that t, t MA in urine of contact workers can be measured by this method.