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目的：研究人胞苷转移酶（CitidyltransferaseCT）cDNA在鼠巨噬细胞中的表达。方法：按照T．Mainiatis等人[1]的方法从转染的巨噬细胞中提取总RNA，电泳分离RNA，转移BNA至尼龙膜并固定，制备32p标记的胞苷转移酶DNA探针，杂文及放射性自显影。结果：对照组无表达，同类转染的细胞均有不同程度的表达。结论：制备探针前应用电泳法确定CTcDNA片段的浓度比紫外光吸收法更准确适用。Quickspin柱（SephedexG-25）用于回收标记的探针，有利于提高探针的纯度，增加杂交的灵敏度。
AIM: To investigate the expression of cDNA of human cytidinetransferase CT in murine macrophages. Method: Follow T. The method of Mainiatis et al [1] extracted total RNA from transfected macrophages, separated RNA by electrophoresis, transferred BNA to nylon membrane and immobilized to prepare 32p-labeled cytidine transferase DNA probe, essay and autoradiography. Results: There was no expression in the control group, and the cells transfected with the same kind of cells showed different degrees of expression. Conclusion: It is more accurate and accurate to determine the concentration of CTcDNA fragments by ultraviolet spectrophotometry before preparing probes. Quickspin column (SephedexG-25) is used to recover the labeled probe, which can improve the purity of the probe and increase the sensitivity of hybridization.