Objective:To study the anti-tumor activities of furanodiene(C15H20O),a primary sesquiterpene compound isolated from the essential oil of the rhizome of Curcuma wenyujin YH Chen et C.Ling(Wen Ezhu),in vitro and in vivo.Methods:In vitro MTT assay was used to further study the effects of time and dosage on anti-proliferation of furanodiene against the sensitive Hela,Hep-2,HL-60,U251 cells,based on the cytotoxic effects of furanodiene on 12 human malignant tumor cell lines with the essential oil of Wen Ezhu as control.,and the half-inhibitory concentration(IC50) was observed.In vivo uterine cervix(U14) tumor cell was selected and the conventional assay method of anti-tumor activity was employed.Furanodiene liposome was administered intraperitoneally,and tumor-inhibitory rate,thymus and spleen indexes were observed.Results:The inhibitive effects on cell proliferation were shown in all of the twelve cell lines and the cytotoxic effects of furanodiene against Hela,Hep-2,HL-60,U251 cells were observed after 12 h of administration,the effect could last for at least 48 h in a dose dependent manner,and the IC50 values were 0.6,1.7,1.8,7.0 μg/ml,respectively.Furanodiene was also found to show inhibitive effects on the proliferation of uterine cervix(U14) tumor induced in mice.The tumor inhibition rates were 36.09%(40 mg/kg),41.55%(60 mg/kg),58.29%(80 mg/kg),respectively.Conclusion:Furanodiene is one of primary anti-cancer active components in the essential oil of Wen Ezhu,and also a very effective agent against uterine cervix cancer,and has protection effect on the immune function. Objective: To study the anti-tumor activities of furanodiene (C15H20O), a primary sesquiterpene compound isolated from the essential oil of the rhizome of Curcuma wenyujin YH Chen et al. (Wen Ezhu), in vitro and in vivo. Methods: In vitro MTT assay was used to further study the effects of time and dosage on anti-proliferation of furanodiene against the sensitive Hela, Hep-2, HL-60, U251 cells, based on the cytotoxic effects of furanodiene on 12 human malignant tumor cell lines with the essential oil of Wen Ezhu as control., and the half-inhibitory concentration (IC50) was observed. In vivo uterine cervix (U14) tumor cell was selected and the conventional assay method of anti-tumor activity was employed. Results: The inhibitive effects on cell proliferation were shown in all of the twelve cell lines and the cytotoxic effects of furanodiene against Hela, Hep-2, HL-60, U251 cells were observed after 12 h of administration, the effect could last for at least 48 h in a dose dependent manner, and the IC50 values ​​were 0.6, 1.7, 1.8, 7.0 μg / ml, respectively. Fuuranodiene was also found to show inhibitive effects on the proliferation of uterine cervix (U14) tumor induced in mice.The tumor inhibition rates were 36.09% (40 mg / kg), 41.55% (60 mg / kg), 58.29% (80 mg / kg), respectively.Conclusion: Furanodiene is one of primary anti-cancer active components in the essential oil of Wen Ezhu, and also a very effective agent against uterine cervix cancer, and has protection effect on the immune function.