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目的探讨丁酸钠(NaB)对CNE2鼻咽癌细胞γ干扰素(IFN-γ)诱导的吲哚胺2,3双加氧酶(IDO)表达调控及分子机制。方法采用Western blot法、免疫共沉淀等方法检测鼻咽癌细胞内IDO的表达及乙酰化、泛素化修饰情况。结果与单独加IFN-γ对照组相比,Western blot结果显示,IFN-γ和Na B联合处理的CNE2细胞IDO蛋白表达增加;免疫共沉淀实验结果表明,联合处理CNE2细胞乙酰化IDO水平和泛素化IDO水平均明显增高;与NaB和IFN-γ联合处理的CNE2细胞相比,NaB、IFN-γ和硼替佐米(bortezomib)联合处理的CNE2细胞的IDO表达量增加。结论 NaB能以剂量依赖性的方式下调鼻咽癌细胞内IFN-γ诱导的IDO表达,IFN-γ和Na B联合处理促进IDO的乙酰化和泛素化。
Objective To investigate the molecular mechanism of sodium butyrate (NaB) on the expression of indoleamine 2,3 dioxygenase (IDO) induced by IFN-γ in CNE2 nasopharyngeal carcinoma cells. Methods Western blot and co-immunoprecipitation were used to detect the expression of IDO, acetylation and ubiquitination in nasopharyngeal carcinoma cells. Results Compared with the IFN-γ alone group, Western blot results showed that the IDO protein expression of CNE2 cells treated with IFN-γ and Na B increased. The results of co-immunoprecipitation showed that the combined treatment of CNE2 cells with acetylated IDO level and pan Compared with CNE2 cells treated with NaB and IFN-γ, the expression of IDO in CNE2 cells treated with NaB, IFN-γ and bortezomib increased significantly. Conclusion NaB can down-regulate the expression of IDO induced by IFN-γ in nasopharyngeal carcinoma cells in a dose-dependent manner. The combination of IFN-γ and Na B can promote the acetylation and ubiquitination of IDO.