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目的组氨酸三聚体核苷结合蛋白1(histidine triad nucleotide-binding protein1,HINT1)基因在喉鳞状细胞癌组织中的表达情况及其与患者临床病理特征的关系,探讨HINT1基因在喉鳞状细胞癌发病过程中的作用。方法通过实时定量PCR和免疫组织化学检测82例喉鳞状细胞癌组织和56例癌旁组织中HINT1mRNA和蛋白水平的表达,分析其蛋白表达与患者临床病理特征的关系。结果喉鳞状细胞癌组织HINT1mRNA水平显著低于癌旁组织(P<0.01),HINT1蛋白表达阳性率显著低于癌旁组织(P<0.05)。晚期喉鳞状细胞癌组织中HINT1蛋白表达显著低于早期喉鳞状细胞癌组织(P<0.05)。结论 HINT1在喉鳞状细胞癌中可能发挥抑癌基因的作用。
Objective To investigate the expression of histidine triad nucleotide-binding protein 1 (HINT1) gene in laryngeal squamous cell carcinoma (LSCC) and its relationship with clinicopathological features, and to explore the relationship between the expression of HINT1 gene in laryngeal squamous cell carcinoma The role of cell carcinoma in the pathogenesis. Methods The expression of HINT1 mRNA and protein in 82 cases of laryngeal squamous cell carcinoma (LSCC) and 56 cases of paracancerous tissues was detected by real-time quantitative PCR and immunohistochemistry. The relationship between the protein expression and the clinicopathological features was analyzed. Results The HINT1 mRNA level in laryngeal squamous cell carcinoma was significantly lower than that in paracancer tissues (P <0.01). The positive rate of HINT1 protein was significantly lower than that in paracancerous tissues (P <0.05). The expression of HINT1 protein in advanced laryngeal squamous cell carcinoma was significantly lower than that in early laryngeal squamous cell carcinoma (P <0.05). Conclusion HINT1 may play a role in tumor suppressor gene in laryngeal squamous cell carcinoma.