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对昆明地区 3种不同生产模式下的香石竹 (DianthuscaryophyllusL .)进行了调查 ,采集样本 146号 ,利用酶联免疫法和电镜检测法对样本感染香石竹病毒的情况进行检测。结果表明昆明地区主要流行的香石竹病毒为香石竹斑驳病毒和香石竹坏死斑点病毒。以带香石竹斑驳病毒的香石竹品种“俏新郎”为实验材料 ,研究了直接剥茎尖法、高温处理结合剥茎尖法和病毒痤处理结合剥茎尖法 3种方法在脱病毒效率和茎尖成苗率的差异。实验结果表明以加热处理结合剥茎尖法脱病毒效果最好 ,0 2mm茎尖脱病毒率可达 77 78% ,加 5 %病毒痤处理对脱病毒有一定的影响 ,直接剥茎尖法脱病毒效果最差。
The samples of Dianthus caryophyllus L. were collected from three different production modes in Kunming. Samples 146 were collected. The samples were infected with carnation by enzyme-linked immunosorbent assay and electron microscopy. The results showed that the predominant carnation virus in Kunming was carnation mottle virus and carnation necrosis spot virus. In this study, the Qiantinglang, a carnation variety with carnation mottle virus, was used as experimental material to study the effects of direct peel-tip method, high-temperature treatment combined with peel-tip method and viral acne treatment with peel- Differences of shoot tip seedling rate. The experimental results showed that the heat treatment combined with the best method to remove the virus out of the tip of the tip, 0 2mm head tip virus-down rate of 77 78%, plus 5% virus acne treatment has a certain impact on virus-free, The virus is the worst.