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目的探讨系统性红斑狼疮(SLE)患者体内血小板相关抗体(PAIgG)水平的变化及其与患者血小板功能的关系。方法采用ELISA方法检测46例SLE患者(病例组)及50例健康体检者(对照组)血清血小板抗体PAIgG和血清血小板α-颗粒膜蛋白-140(GMP-140)水平;采用比浊法测定两组患者血小板活化指标二磷酸腺苷(ADP)诱导的血小板聚集率;采用流式细胞学方法测定血小板激活复合物-1(PAC-1)表达率。分析患者血清PAIgG水平与血小板数量和功能的关系。结果病例组血小板数量较对照组显著下降(t=-8.125,P<0.01),而PAIgG水平,GMP-140水平,PAC-1表达率均显著高于对照组(t=-5.264、-3.244、-2.331,P<0.05)。相关性研究表明,病例组患者PAIgG水平与血小板数量呈负相关性(r=-0.223,P<0.01),而与GMP-140浓度及PAC-1表达率水平呈正相关性(r=0.532,P<0.05;r=0.614,P<0.05)。结论 SLE患者体内血清抗血小板抗体PAIgG水平显著增加,血小板数量下降。PAIgG水平的变化可能与血小板活化,聚集力增加有关。
Objective To investigate the changes of platelet-associated antibody (PAIgG) in patients with systemic lupus erythematosus (SLE) and its relationship with platelet function. Methods Serum platelet antibody (PAIgG) and serum platelet-α-granule membrane protein-140 (GMP-140) levels in 46 SLE patients and 50 healthy controls were measured by ELISA. The levels of GMP- The platelet aggregation rate induced by adenosine diphosphate (ADP) was measured in the platelet of patients. The expression of platelet-activating complex-1 (PAC-1) was measured by flow cytometry. PAIgG levels in patients with serum platelet count and function of the relationship. Results The number of platelets in the case group was significantly lower than that in the control group (t = -8.125, P <0.01), while the levels of PAIgG, GMP-140 and PAC-1 were significantly higher than those in the control group -2.331, P <0.05). Correlation studies showed that PAIgG level was negatively correlated with platelet count (r = -0.223, P <0.01), but positively correlated with GMP-140 concentration and PAC-1 expression level (r = 0.532, P <0.05; r = 0.614, P <0.05). Conclusion The level of serum anti-platelet antibody PAIgG in SLE patients increased significantly and the number of platelets decreased. PAIgG level changes may be related to platelet activation, increased aggregation.