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目的探讨洗脱法、直接投入法和涂抹法这三种样本制作方法对消毒监测中的无菌检验结果的影响。方法采集2016年5月1日至2016年10月25日内黄县医疗单位综合监测中的一次性医疗用品及一次性工作衣帽、口罩共计445份样本数,分别用洗脱法、直接投入法、涂抹法制作样本,观察不同制作方法的样本菌培养阳性率。结果此次共采集样本445份,360份进行洗脱法和直接投入法的比较,直接投入法阳性率为4.44%,洗脱法阳性率为8.33%;85份进行直接投入法和涂抹法的比较,直接投入法阳性率9.41%,涂抹法阳性率20.00%。结论在一次性医疗用品的卫生检验中,采用洗脱法和直接投入法来制作样本,做无菌检验试验,检验结果有明显差异,此类样本宜用直接投入法进行样本制作;用直接投入法和涂抹法来进行一次性工作衣帽、敷料等非管道样本制作方法对无菌实验结果影响的比较。检验结果差异有统计学意义。说明在一次性工作衣帽、敷料等非管道样本的样本制作中应尽量采用涂抹法采样。
Objective To investigate the effects of three methods of sample preparation such as elution, direct injection and smear on the results of sterility test in disinfection monitoring. Methods A total of 445 samples of disposable medical products and one-time working clothing and disposable masks were collected from May 1, 2016 to October 25, 2016 in the comprehensive monitoring of medical units in Huang County. The data were analyzed by elution method and direct investment method , Smear method to make samples and observe the positive rate of sample culture in different production methods. Results A total of 445 samples were collected, 360 samples were compared with the direct injection method, the direct injection method positive rate was 4.44%, the positive rate of elution method was 8.33%; 85 direct injection method and smear method Comparison, direct input method positive rate of 9.41%, smear positive rate of 20.00%. Conclusions In the hygiene inspection of disposable medical products, the samples were made by the elution method and the direct input method, so as to make the sterility test. The results of the test are obviously different. Such samples should be made by direct input method. Method and smear method to make a one-time work The comparison of the influence of non-pipe sample making methods on the results of the aseptic experiment, such as the dressing and dressing. The test results were statistically significant. Explain that in one-time working clothes, dressings and other non-pipe sample samples should try to use smear sampling.