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为提高单克隆抗体制备过程中杂交瘤细胞的融合率,本文作者采用了新的融合技术-电融合法(Electro fusion)取代传统的PEG法,并成功地获得一株抗细胞角蛋白单克隆抗体的细胞株(A53 B/A2).作者用小鼠浆细胞瘤细胞系X63 Ag8.653作为亲本细胞与经MCF 7细胞免疫后的小鼠脾细胞进行融合.收集的脾细胞先经渗透压休克处理以除去红细胞.X63 Ag8.653经蛋白酶处理后,加入脾细胞.两种
In order to improve the fusion rate of hybridoma cells during the preparation of monoclonal antibodies, the authors adopted a new fusion technology - Electro fusion instead of the traditional PEG method and successfully obtained a monoclonal antibody against cytokeratin (A53 B / A2) .A mouse plasmacytoma cell line X63 Ag8.653 was used as a parent cell to fuse with mouse spleen cells after MCF 7 cell immunization.The collected splenocytes were pre-treated with osmotic pressure shock Treated to remove red blood cells.X63 Ag8.653 after protease treatment, spleen cells were added.